tag:blogger.com,1999:blog-32079676.post1148201139808708658..comments2024-02-13T21:22:02.522-08:00Comments on RRResearch: New microarray dataRosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-32079676.post-5640483629074059682008-01-27T07:23:00.000-08:002008-01-27T07:23:00.000-08:00Nice idea. But we're in a bit of a bind with resp...Nice idea. But we're in a bit of a bind with respect to binding sites or active sites as we still don't know what Sxy binds to or how it acts, and the only null mutants we have are insertional knockouts. We still need to do a screen for point mutations that affect function.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-21751264286834467562008-01-19T19:24:00.000-08:002008-01-19T19:24:00.000-08:00I can't remember what you've said about sxy in the...I can't remember what you've said about sxy in the past. Can you make a null version of the protein (mutated active site/binding site/something essential) and overexpress that to test for effects of pure overexpression?iayorkhttps://www.blogger.com/profile/10717810444752993915noreply@blogger.com