tag:blogger.com,1999:blog-32079676.post2426694551124429411..comments2024-02-13T21:22:02.522-08:00Comments on RRResearch: Nanotechnology?Rosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-32079676.post-44952054487919018902007-02-22T21:34:00.000-08:002007-02-22T21:34:00.000-08:00Well, I don't know enough about USS uptake to quit...Well, I don't know enough about USS uptake to quite understand your experiment. But I do know a lot about optical tweezers as well as applying forces to single-DNA tethers with magnetic beads.<BR/><BR/>Why would you say that optical tweezers do not have the distance precision for your experiment? Optical tweezers have been shown to resolve individual 0.3 nanometer steps by RNAP polymerase (Block lab). In your case, you would likely have lower resolution, but it would still probably be good enough for measuring partial displacement of the 220 bp fragments. Also, why are the 50 nm beads magnetic? If you are planning on applying forces to them, the force will likely be pretty small, unless you can get a magnet very very close (like 10's of microns). The forces would likely be femtonewton level, which is enough to move free beads around, but wouldn't be significant compared with the molecular motors in the cell. Or, maybe you are just using the magnetic beads first to purify the DNA and then to simply block uptake which would be fine. But in my experience, streptavidin coated magnetic beads are less reliable than nonmagnetic, so you wouldn't want the magnetic unless you were using it.TestingTFVhttps://www.blogger.com/profile/03189943558834286023noreply@blogger.com