tag:blogger.com,1999:blog-32079676.post3614192301747516380..comments2024-02-13T21:22:02.522-08:00Comments on RRResearch: Getting ready for some arsenic dataRosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-32079676.post-86880672246168380322012-01-02T12:17:06.071-08:002012-01-02T12:17:06.071-08:00Are you worried about the possibility that some am...Are you worried about the possibility that some amount of arsenate might be bound (non-covalently) to the DNA?<br /><br />If I remember correctly, one of the problems with the Science paper was that they failed to carefully purify the DNA, leaving open the possibility that arsenate was just 'stuck' non-specifically to the DNA. While the ethanol precipitation and CsCl gradient are more rigorous than the techniques they used, if the arsenate is 'sticky' (poly-saccharide bound?), is it a least plausible that you might observe more arsenate than expected in the DNA peak? <br /><br />I suppose this will all get cleaned up in the mass spec step though --even if there's arsenate in there, if it is getting integrated into the DNA backbone, you'd expect to see fragments coming out that are consistent with arsenate-carbon compounds, right? Isn't that how MALDI-TOF works?Dougnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-80004272517185599482012-01-02T10:33:51.906-08:002012-01-02T10:33:51.906-08:00what's the difference between green and red? a...what's the difference between green and red? any significance the dna samples running at different positions in the gradient?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-87144858243670217122012-01-01T16:35:34.012-08:002012-01-01T16:35:34.012-08:00regarding the soluble contaminants, can you do som...regarding the soluble contaminants, can you do some kind of precipitation to remove contaminating arsenic (i dont know if arsenic precipitates with ethanol or isopropanol)Anonymoushttps://www.blogger.com/profile/12291507118158076247noreply@blogger.com