tag:blogger.com,1999:blog-32079676.post6352138253269452102..comments2024-02-13T21:22:02.522-08:00Comments on RRResearch: A refresher class on streaking for single coloniesRosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger7125tag:blogger.com,1999:blog-32079676.post-65304609555665315432012-03-30T13:10:42.840-07:002012-03-30T13:10:42.840-07:00I use capillary tubes. cheap and smooth.I use capillary tubes. cheap and smooth.bullborehttps://www.blogger.com/profile/03381301042448251386noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-14784014568289247602012-03-26T09:01:45.969-07:002012-03-26T09:01:45.969-07:00We have a large supply of 0.1 ml glass pipettes wh...We have a large supply of 0.1 ml glass pipettes which I use in the same way.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-58100333347546423652012-03-26T09:00:51.868-07:002012-03-26T09:00:51.868-07:00Added!Added!Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-54308768086610668882012-03-26T05:29:00.540-07:002012-03-26T05:29:00.540-07:00I recommend the wooden dowel for isolations (we us...I recommend the wooden dowel for isolations (we use them for both yeast and bacteria). You can get 2 sterile passes for each one by simply rolling the dowel over between passes. You do not flame them so there's no chance of heat shocking (or outright killing) many of the cells and you can simply autoclave them over and over so you limit waste from the disposable loops.The Loraxhttps://www.blogger.com/profile/13361004494346338824noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-36592435890404455132012-03-22T05:01:36.524-07:002012-03-22T05:01:36.524-07:00There's an interesting interpretation of plate...There's an interesting interpretation of plate "streaking" in the second slideshow of microbial art here:<br /><br />http://www.microbialart.com/galleries/niall-hamilton/Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-57538700374560678532012-03-21T15:24:09.205-07:002012-03-21T15:24:09.205-07:00Thank you for this!
How about a photo of a pretty...Thank you for this!<br /><br />How about a photo of a pretty one too? :)Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-50943362346686074132012-03-21T12:32:21.591-07:002012-03-21T12:32:21.591-07:00Another problem is when doesn't sterilise thei...Another problem is when doesn't sterilise their loops properly in between streaks, so bacteria left on the loop are re-streaked.<br />Admittedly, when using disposable plastic loops, you can feel like a spod going through a whole pack of them just for streaking out some transformants that may not have your construct in the right orientation anyway.<br /> One can alternate the side of the loop for streaks if you have the steady hand of a sniper.<br /><br />But this here looks like a basic rookie error. When you start out streaking out practically invisible bacteria, there is always that tiny niggling fear. That the small section of the first pass actually had no bacteria at all, and all one is doing is irritating the surface of the plate, and no streaking has happened at all. So rookies overcompensate on their first few tries.The Defective Brainhttps://www.blogger.com/profile/04753079302510607981noreply@blogger.com