tag:blogger.com,1999:blog-32079676.post6451556726783130322..comments2012-01-25T09:37:33.345-08:00Rosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-32079676.post-38140512810411733612012-01-25T09:37:33.345-08:002012-01-25T09:37:33.345-08:00I avoid this problem by loading the gel and running the samples in for about 20 minutes in a 'non-submarine' manner (i.e. with the running buffer making electrical contact with the ends of the gel but not overlaying it). Once the samples have migrated into the gel, it's safe to add more buffer and run submarine.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-54793836945898917372012-01-25T08:13:52.254-08:002012-01-25T08:13:52.254-08:00I'm curious how did it work out?Nausicaahttp://www.blogger.com/profile/02336112452328911405noreply@blogger.com