Comments on RRResearch: Generating final data for the #arseniclife paper

I think the W-S et al. DNA preps migrated differen...

2012-01-14T19:46:50.458-08:00

I think the W-S et al. DNA preps migrated differently because the +P lane had so much more RNA (and probably DNA) than the -P lane. The RNA in the +P lane had swept up all the EthBr in its path, so the DNA is only stained at the edges of the lane and has the streakiness that's characteristic of overloaded high-molecular-weight DNA. There could also have been differences in how much salt was in the different preps, as they had only been partially purified.

p.s. The gel in this post is the experiment that tests the troll's concern.

did we ever get to the bottom of why felisa's ...

2012-01-14T15:32:08.451-08:00

did we ever get to the bottom of why felisa's +as dna preps migrated differently from her -as in the agarose gel (ignoring the rRNA contamination) whereas your +/- as preps looked identical? is it in any way possible that her dna in the +as contained arsenic and had undergone some hydrolysis and thus was resembling your hypothetical agarose gel experiments you posed a few posts back in response to the 'troll' — just seems like there are some differences between her preps and yours, and wouldn't want those differences to be the difference between detecting As and not detecting it

Thanks so much for keeping us in the loop with the...

2012-01-14T13:12:57.222-08:00

Thanks so much for keeping us in the loop with the #arseniclife story. It's been a fascinating read over the last year as well as your detailed thoughts over your own research topics!