tag:blogger.com,1999:blog-32079676.post7171767494530475884..comments2024-02-13T21:22:02.522-08:00Comments on RRResearch: Estimates of fraction competent aren't very reliableRosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger6125tag:blogger.com,1999:blog-32079676.post-17236546107064666272013-03-13T00:45:17.542-07:002013-03-13T00:45:17.542-07:00Your research about combining them is look awesome...Your research about combining them is look awesome, I don't really knew about DNA. But your research make me understand how difficult to be scientist to create something useful for human life. I will appreciate your research this means something for me.Takekawahttp://tipstobeauty.com/noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-79556142518762786712013-01-28T01:57:58.296-08:002013-01-28T01:57:58.296-08:00Hi Rosie,
Long time, no post. Is this blog dead n...Hi Rosie,<br /><br />Long time, no post. Is this blog dead now? I hope I everything is okay and that you'll start writing again.<br /><br />In any case, I just wanted to say that I've really enjoyed reading your blog over the last couple of years and that it has been really useful and insightful for me as someone who's just now starting a PhD. Jacknoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-39899062008553825552012-12-23T09:57:46.258-08:002012-12-23T09:57:46.258-08:00Hi Rosie,
it is always great to read your blog.
D...Hi Rosie,<br />it is always great to read your blog.<br /><br />Do you have cell division during those 1.5 hours after adding the map7 DNA? In which case perhaps frequencies are not very accurate and you would need a Luria-Delbruck kind of analysis for more precise estimates? Perhaps one that thakes into account that some resistance genes cause changes in growth rate.<br /><br />Lilia<br />Dr Lilhttps://www.blogger.com/profile/12182596096133463254noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-90486000164952408172012-12-19T04:57:10.242-08:002012-12-19T04:57:10.242-08:00good post. Relps me by writing my thesisgood post. Relps me by writing my thesisSlapimo tyrimaihttp://www.sosdiagnostika.lt/slapimo-tyrimainoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-22446471415246582862012-12-10T16:32:30.267-08:002012-12-10T16:32:30.267-08:00For the last few weeks, the blue text of your post...For the last few weeks, the blue text of your posts has appeared "behind" the almost opaque green background and has been practically unreadable--to this Mac OSX/Safari user at least.<br /><br />Can you fix it, please?John Parkhttp://web.ncf.ca/af250/JMPHome.htmlnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-24218894291032860042012-11-17T11:29:40.756-08:002012-11-17T11:29:40.756-08:00If fluorescent reporters are reliable readouts of ...If fluorescent reporters are reliable readouts of transformation, you could use flow cytometry to count fluorescent cells -- more reliable, objective, and high-throughput than microscopy. <br /><br />But I question whether fluorescence would be a great way of measuring transformation. That assumes that all the cells that take up plasmid express fluorescence at the some kinetics and intensity. I have no experience with this for bacterial cells, but mammalian cells show a wide distribution of fluorescence intensity this way, in my experience. You might end up measuring many parameters layered on top of actual transformation.iayorkhttps://www.blogger.com/profile/10717810444752993915noreply@blogger.com