tag:blogger.com,1999:blog-32079676.post754741929287910443..comments2012-01-20T16:15:19.773-08:00Rosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-32079676.post-89358348775139585772012-01-20T16:15:19.773-08:002012-01-20T16:15:19.773-08:00Forgot to say: Wolfe-Simon et al. didn&#39;t provide any information about their reagents, so I used ordinary reagent-grade chemicals from our usual suppliers (Fisher, Sigma, whatever we had on our shelves). As my new post explains, our media did appear to have less phosphate contamination than theirs did, which is why I added an additional 3 µM phosphate to my &#39;P-&#39; cultures.Rosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-3828894040959790812012-01-20T16:02:01.181-08:002012-01-20T16:02:01.181-08:00The Nature article was wrong - I do see growth in AML60 medium without added phosphate. I think I&#39;d better do a new post about this concern.Rosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-80672325243291986352012-01-20T15:05:38.045-08:002012-01-20T15:05:38.045-08:00I just stumbled across your blog through the article currently posted on the Nature website (which I now plan to follow), so I am not completely up to date on your results and approach to looking at this issue, but I am curious to know if you are use the same reagents (brand, catalog #, etc) as the authors of the article in science? <br /><br />I ask because I am currently conducting growth studies pertaining to potassium in a halophilic archaeon and have found that my minimal obtainable potassium concentration in my medias vary widely with the brand of high-purity salts I use. For example, with Fluka high-purity NaCl (&gt;99.9999% by trace metal analysis) I still have a final potassium concentration of approximately 8mM. Could this be the reason you aren&#39;t seeing growth without the addition of 3uM phosphate and they did? Perhaps you are using higher purity reagents?<br /><br />Just a thought.Mattnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-38532099070113429042012-01-17T21:50:32.628-08:002012-01-17T21:50:32.628-08:00I am enjoying myself (astrobiology interest) with catching up on #arseniclife, well #noarseniclife now, and have to pitch in with others yet again with hearth felt thanks for doing this (and in public)! Looking forward to see the Brevia publication.Torbjörn Larsson, OMhttp://www.blogger.com/profile/13304729731231255545noreply@blogger.com