tag:blogger.com,1999:blog-32079676.post7745262422104412210..comments2024-02-13T21:22:02.522-08:00Comments on RRResearch: Why my gels wouldn't set?Rosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger6125tag:blogger.com,1999:blog-32079676.post-3058093391331521632012-05-16T23:59:16.030-07:002012-05-16T23:59:16.030-07:00I make 10 % APS solutions (10 ml) and make 1 ml al...I make 10 % APS solutions (10 ml) and make 1 ml aliquotes and store at -20C. Keep the thawed tube at 4C till it get used up. This works great for me. What is the recipe for your gel? I use the Sambrook recipe and it works fine.Grad Studenthttps://www.blogger.com/profile/15092682341296306845noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-25008003796780639952012-05-16T01:48:39.954-07:002012-05-16T01:48:39.954-07:00Ah yes, casting gels for the first time after year...Ah yes, casting gels for the first time after years. I tried that recently, using a recipe my grad student gave me. I was too daft to realize that he uses a far more dilute APS solution than I always make. My gels set the moment I tried to pour them. Luckily it was late, so only about ten people saw me...Thomas Barendshttps://www.blogger.com/profile/02563436899930811698noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-14140265069225545342012-05-15T18:41:34.449-07:002012-05-15T18:41:34.449-07:00@Rosie: 10% APS can hang out for up to three month...@Rosie: 10% APS can hang out for up to three months at 4 degrees. But dry APS can go off too. Keep it in a dessicator if you don't already - once it starts looking wet, it's done.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-52630004097933044452012-05-15T16:40:59.518-07:002012-05-15T16:40:59.518-07:00LOL. This happened to me in the Summer.LOL. This happened to me in the Summer.Tylerhttp://www.tylersuzukinelson.comnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-17852405731360758982012-05-15T10:57:07.245-07:002012-05-15T10:57:07.245-07:00I make the APS (ammonium persulfate) fresh, mainly...I make the APS (ammonium persulfate) fresh, mainly because that's what I was taught and I don't know enough about the chemistry to decide how long it would keep at room temperature or frozen.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-30082105634641184072012-05-15T10:44:05.316-07:002012-05-15T10:44:05.316-07:00Make sure your APS hasn't gone to hell either....Make sure your APS hasn't gone to hell either.Anonymousnoreply@blogger.com