tag:blogger.com,1999:blog-32079676.post864906914575236044..comments2024-02-13T21:22:02.522-08:00Comments on RRResearch: More cell preps for RNA-seq analysesRosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-32079676.post-51050652402911101562014-06-14T04:03:33.513-07:002014-06-14T04:03:33.513-07:00If it's cheap I'm interested, since we'...If it's cheap I'm interested, since we're almost broke!Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-3778427129365014072014-06-14T03:25:25.861-07:002014-06-14T03:25:25.861-07:00Hi Rosie, why not use higher numbers of replicates...Hi Rosie, why not use higher numbers of replicates, and the same number of replicates in each group? From experience you're much more likely to detect differential expression with four replicates; five or six is best. If this is an RNA-seq experiment you are planning then at four reps for each group youd have 20 samples which when multiplexed ina v4 SE50 HiSeq lane would give 10-15M reads each, perfect for DE. You may be interested in this post I wrote: <a href="http://core-genomics.blogspot.co.uk/2014/03/cheaper-rna-seq-but-you-might-have-to.html" rel="nofollow">cheap RNA-seq</a>.<br /><br />PS: I'm a real fan of how your group uses the blog.James@cancerhttps://www.blogger.com/profile/02825715598810395734noreply@blogger.com