tag:blogger.com,1999:blog-32079676.comments2024-02-13T21:22:02.522-08:00RRResearchRosie Redfieldhttp://www.blogger.com/profile/06807912674127645263noreply@blogger.comBlogger2142125tag:blogger.com,1999:blog-32079676.post-36360806322737075552024-02-13T18:55:41.803-08:002024-02-13T18:55:41.803-08:00Yeah we do the microwave. And it means that there&...Yeah we do the microwave. And it means that there's a person in the lab who once a month or so has to clean the microwave to get all the assorted stuff that boiled over and didn't get properly cleaned by the person heating it. I really wish that more people's parents had taught them to clean up after themselvesMichael Phillipshttps://www.blogger.com/profile/15926937158452518195noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-74637940573374419902024-02-12T12:25:21.081-08:002024-02-12T12:25:21.081-08:00For a long time now I've used a 1N solution of...For a long time now I've used a 1N solution of NaOH to make hemin stocks for anaerobic culture media (5 to 10 mg/ml). I filter sterilize with 0.22 micron PES membrane filter and store it refrigerated in the dark for up to 6 months. Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-73453152156469430632022-10-27T13:16:12.581-07:002022-10-27T13:16:12.581-07:00İlginç 😊İlginç 😊Hadımköy çilingirhttp://www.hadimoycilingir.comnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-49693763327606046012022-10-04T04:14:43.992-07:002022-10-04T04:14:43.992-07:00Hastalık diye birşey yok insanlar ke di kendilerin...Hastalık diye birşey yok insanlar ke di kendilerini hata ediyorlar Beylikdüzü Çilingirhttp://www.beylikduzucilingirci.comnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-68712710645739768252022-10-04T04:12:13.436-07:002022-10-04T04:12:13.436-07:00:(((( tuhafff:(((( tuhafffbeylikdüzü çilingirhttp://www.beylikduzu.cilingircisi.comnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-13537763956997180602022-09-17T15:36:06.960-07:002022-09-17T15:36:06.960-07:00are you still maintaining your position?are you still maintaining your position?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-23293480476510004802022-09-16T10:13:32.693-07:002022-09-16T10:13:32.693-07:00Hi rosie.. I added 300 microlitre of 0.5mg/ml etbr...Hi rosie.. I added 300 microlitre of 0.5mg/ml etbr to 500 ml gel.. due to mishandling i was exposed to vapours for atleast 5 min.. M feeling skin irritation on face since 2 days.. Was this conc too high..do i need to worry..? Pls let me know.. What should be the amount of etbr added to 3% agarose 500ml?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-45656082566780762732020-11-17T11:26:56.828-08:002020-11-17T11:26:56.828-08:00Hi.I'm very interesting about your thesis.I...Hi.I'm very interesting about your thesis.I'd like to get in contact with you. Is It possibile? Write me on ginevrix@gmail.com marco pretellihttps://www.blogger.com/profile/10290613644062587664noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-83821635540610263912020-06-23T12:28:30.569-07:002020-06-23T12:28:30.569-07:00Hello, Rosie. Thanks for this article.
I'm an...Hello, Rosie. Thanks for this article. <br />I'm an undergrad student and I worked for a year in a molecular biology lab. I worked with EB stained gel several times (always wearing gloves, of course).<br />Lately, thought, I've become paranoid. I was convinced I might have a bit of it on my clothes and I was worried sick that it would've contaminated my entire wardrobe (I know, I know. Yes, I'm way too paranoid, yes, I'm working on it.) I even pestered one of my ex-colleagues asking if I should be worried.<br />Anyway, thanks, really. I'm not 100% tranquilized but at least I feel calmer.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-59640302386976452752020-06-05T12:26:55.154-07:002020-06-05T12:26:55.154-07:00Thank you Rosie! Saved me a headache!Thank you Rosie! Saved me a headache!Jasonhttps://www.blogger.com/profile/16240429021136697268noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-42544639332527077172020-04-26T07:59:22.370-07:002020-04-26T07:59:22.370-07:00Sure. Post here, and if I don't answer then e...Sure. Post here, and if I don't answer then email me.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-54731966042183748612020-04-25T23:20:09.334-07:002020-04-25T23:20:09.334-07:00Very clear and illustrated explanation. I am carry...Very clear and illustrated explanation. I am carrying out a theoretical study on a diy bacteriophage production system and I seek to understand in detail all the issues. Not having access to standardized products and protocols, this forces me to understand the details of what exactly happens in the phage bacteria interaction. Could I ask you a few questions for which I can't find an answer?<br />Sincerly Yann Rouxelyrouxelhttps://www.blogger.com/profile/10532753383975772344noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-19233391461962915912020-03-17T11:10:32.768-07:002020-03-17T11:10:32.768-07:00Forgot to add Happy Patrick's Day!Forgot to add Happy Patrick's Day!Anonymoushttps://www.blogger.com/profile/14537349029203099849noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-38101754071227535322020-03-17T11:09:59.741-07:002020-03-17T11:09:59.741-07:00Thank you very much for this Doc. Please stay heal...Thank you very much for this Doc. Please stay healthy, we need to keep the coolest professor on the planet! Anonymoushttps://www.blogger.com/profile/14537349029203099849noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-14793120929757438242020-03-17T06:55:43.436-07:002020-03-17T06:55:43.436-07:00This is my fear, that we are only delaying the ine...This is my fear, that we are only delaying the inevitable, at a massive financial cost. I think that slowing things down for the next few weeks, allowing time for health care preparations, makes sense, but shutting down for six months is just not practical. I don't think we are thinking far enough ahead.Laurihttps://www.blogger.com/profile/05584157319278511653noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-42699002497439611442020-03-16T15:55:19.519-07:002020-03-16T15:55:19.519-07:00You seem to have only started moderate action in w...You seem to have only started moderate action in week 2, high cost action in week 5, and extreme action in week 8, each time after ramping through the others. Why?<br /><br />What happens if you start extreme action as soon as possible? I would assume you extinguish the infection sooner, which may in turn buy you time to set up screening that effectively prevents re-seeding from imported cases.Peterhttps://www.blogger.com/profile/12559721137290332762noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-1100181293097348352019-10-09T14:02:10.138-07:002019-10-09T14:02:10.138-07:00No.The chance that the ethidium you've been ex...No.The chance that the ethidium you've been exposed to would harm you is very tiny - much less than, say, 5 minutes of sunshine.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-21375575995098115502019-10-09T13:54:52.708-07:002019-10-09T13:54:52.708-07:00Hello. I toouched the frame into which the buffer ...Hello. I toouched the frame into which the buffer was poured. I washed the frame without gloves under the water. Now I am worrying about it a long time. Next time my colleague heated the agarose gel with ethidium bromide. It is dangerous for me?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-16567120323963614682019-10-09T13:52:40.179-07:002019-10-09T13:52:40.179-07:00No.No.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-76490318247774455992019-10-09T13:52:01.869-07:002019-10-09T13:52:01.869-07:00This comment has been removed by the author.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-77344416383892623412019-10-09T13:50:24.489-07:002019-10-09T13:50:24.489-07:00Hello. I touched a tray that the buffer is pouring...Hello. I touched a tray that the buffer is pouring into with EtBr. Do I need to worry?Anonymoushttps://www.blogger.com/profile/17878620213505647952noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-7488469136424558822019-08-14T10:33:04.213-07:002019-08-14T10:33:04.213-07:00IANAC (I am not a chemist) but I doubt it.IANAC (I am not a chemist) but I doubt it.Rosie Redfieldhttps://www.blogger.com/profile/06807912674127645263noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-89272778067674862462019-08-14T01:35:44.211-07:002019-08-14T01:35:44.211-07:00Is there any solid lipid that can dissolve hemin?Is there any solid lipid that can dissolve hemin?Anonymoushttps://www.blogger.com/profile/07712535977969094713noreply@blogger.comtag:blogger.com,1999:blog-32079676.post-47276970085359559042019-06-27T06:22:00.650-07:002019-06-27T06:22:00.650-07:00I cleaned up ethidium bromide with bleach once bec...I cleaned up ethidium bromide with bleach once because I read that was the correct way to clean it up.Sarahnoreply@blogger.comtag:blogger.com,1999:blog-32079676.post-42119008583972474962018-05-21T09:41:12.907-07:002018-05-21T09:41:12.907-07:00Hi Bill, do you need to just mix the reagents or d...Hi Bill, do you need to just mix the reagents or do you need to adjust Ph like other recipes do. Also, some recipes do not include boric acid, why do you need to include it? The other recipes where just basically mixing sodium borate tetrahydrate with water...Juan Pablo Aguilarhttps://www.blogger.com/profile/18092152623925277189noreply@blogger.com