Phage phrustration

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Aacckk!  I've spent more than a month trying to get decent R. capsulatus phage plaques on R. capsulatus lawns.  Still no consistent su...

Phage plans - let's put natural selection to work!

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I have a two-pronged plan to get a phage strain that gives good enough plaques for my GTA-as-vaccine experiments. I obtained reasonable ti...

thin lawns, feeble or absent phage

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My phage titering gave disappointing results.  Three of the five lysates gave no plaques at all, and the other two gave small indistinct pl...

Titering my lysates

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Planning today's work: Titering the phage lysates should be a no-brainer, but it's been a long time since I worked with phage so I...

About bacterial lawns and phage plaques

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This was going to be a post where I do the planning to titer my new lysates today, but it turned into an explanation of how microbiologists ...
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Questions about CRISPR-mediated phage immunity

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Thursday's post described the hypothesis that bacteria might use gene transfer agent particles to inoculate other cells in the populati...

Why GTA genes can't be maintained by 'selfish' transmission

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Below is the line of reasoning showing that the genes responsible for producing GTA particles cannot maintain themselves or spread into new ...

Might GTA be a vaccination system for infecting phages?

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My work at Dartmouth (to be described in upcoming posts) showed conclusively that genes encoding Gene Transfer Agents (such as the GTA syste...

Model of GTA evolution by infectious transfer

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Here's the description of my model addressing Explanation 1 for GTA persistence.  For now I've just pasted in the text of a Word fil...