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in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
Not the result I was hoping for....
This is beta-galactosidase activity in cultures that have been transferred from LB (= rich medium) to M9 salts plus a tiny bit of casamino acids (starvation medium). Transfer was at time=0.
All cells carried the ppdA::lacZ fusion on the same plasmid. The black line is cells that are sxy+ and crp+. The blue line cells are sxy-, and the red line cells are crp-.
It's clear that beta-gal activity increased similarly in all three cultures. As seen for the cultures in rich medium (previous post), the crp- cells had slightly lower activity, while the sxy+ and sxy- cells had almost identical activity.
So I conclude that the treatments I've tried so far have not changed the Sxy-dependent activity of the ppdA promoter.
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