I was about to order a tester-kit of streptavidin-coated magnetic beads from Invitrogen (4 different bead surface types, to find the surface that gives the least background), when I realized that these weren't the tiny 50 nm beads I had in mind but 1 µ beads that are as big as H. influenzae cells. I don't want to use great big beads if I can get smaller ones (yes, I realize that 1 µ is hardly 'great big').
Now I can't find the supplier that had the 50 nm streptavidin-coated beads at all. The best I can find is a European company, Ademtech, that has 100 and 200 nm beads. I've sent them an email asking if they have a Canadian supplier, but I'm not optimistic. Maybe I should just try the tester kit of big beads, and then scale down...
Invitrogen charges more than $500 for a stand that holds 16 microfuge tubes against rare-earth magnets, to separate the beads from the liquid they're suspended in. The 6-tube one is only about $150. But, I can buy a sampler of 50 NdFeB magnets of assorted sizes (1/8 inch to 1/2 inch, rods and discs) from Lee Valley Tools for $16.50.
- Home
- Angry by Choice
- Catalogue of Organisms
- Chinleana
- Doc Madhattan
- Games with Words
- Genomics, Medicine, and Pseudoscience
- History of Geology
- Moss Plants and More
- Pleiotropy
- Plektix
- RRResearch
- Skeptic Wonder
- The Culture of Chemistry
- The Curious Wavefunction
- The Phytophactor
- The View from a Microbiologist
- Variety of Life
Field of Science
-
-
-
Political pollsters are pretending they know what's happening. They don't.5 weeks ago in Genomics, Medicine, and Pseudoscience
-
-
Course Corrections6 months ago in Angry by Choice
-
-
The Site is Dead, Long Live the Site2 years ago in Catalogue of Organisms
-
The Site is Dead, Long Live the Site2 years ago in Variety of Life
-
Does mathematics carry human biases?4 years ago in PLEKTIX
-
-
-
-
A New Placodont from the Late Triassic of China5 years ago in Chinleana
-
Posted: July 22, 2018 at 03:03PM6 years ago in Field Notes
-
Bryophyte Herbarium Survey7 years ago in Moss Plants and More
-
Harnessing innate immunity to cure HIV8 years ago in Rule of 6ix
-
WE MOVED!8 years ago in Games with Words
-
-
-
-
post doc job opportunity on ribosome biochemistry!9 years ago in Protein Evolution and Other Musings
-
Growing the kidney: re-blogged from Science Bitez9 years ago in The View from a Microbiologist
-
Blogging Microbes- Communicating Microbiology to Netizens10 years ago in Memoirs of a Defective Brain
-
-
-
The Lure of the Obscure? Guest Post by Frank Stahl12 years ago in Sex, Genes & Evolution
-
-
Lab Rat Moving House13 years ago in Life of a Lab Rat
-
Goodbye FoS, thanks for all the laughs13 years ago in Disease Prone
-
-
Slideshow of NASA's Stardust-NExT Mission Comet Tempel 1 Flyby13 years ago in The Large Picture Blog
-
in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
1 comment:
Markup Key:
- <b>bold</b> = bold
- <i>italic</i> = italic
- <a href="http://www.fieldofscience.com/">FoS</a> = FoS
Subscribe to:
Post Comments (Atom)
Have you tried Miltenyi Biotec? They have 50nm magnetic beads and ship to Canada. I've only used the GFP ones but they work great.
ReplyDelete