I just reread an opinion piece from Richard Moxon's group, considering whether H. influenzae produces anything that should really be called a biofilm. They agree that H. influenzae cells will grow on surfaces, but they don't think there's any real evidence that these films are the result of a developmental program that evolved because of the benefits of biofilm formation.
Although 'biofilm' can simply mean a layer of bacterial cells and biological macromolecules, associated with a solid surface or an interface, most microbiologists assume that biofilms arise by regulated developmental programs. Said another way, most microbiologists think that bacteria grow in biofilms because natural selection has favoured genes that cause them to do so. They think that bacteria respond to certain stresses that arise in biofilms, or simply to the presence of a surface, by turning on sets of genes that optimize their ability to initiate a biofilm and the physical properties of the biofilm that forms.
It's not that researchers have rigorously evaluated and discarded the alternative explanation - that biofilms form just because bacteria have adhesive organelles and macromolecules are often sticky and fibrous. But they, and grant agencies and journal editors, find the adaptive perspective more interesting, so that's what gets done and published. The Moxon paper is complaining that the term biofilm, with its aura of scientific coolness, is being applied to H. influenzae structures that fit only my simple non-adaptive definition.
Thus at least part of the reason why my simple DNA-on-glass-tubes experiment didn't work is probably that the H. influenzae cells were never going to form a biofilm at all.
After we discussed this at lab meeting, I'm going to try something different. I'll make a fake biofilm by putting a layer of top agar that contains DNA on top of normal agar plates, and then testing whether cells stick to the surface. I'll also return to an experiment I did by accident when I was a post-doc, which showed that multiple cells can bind to single DNA fragments in solution.
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