The RA's gone off on a short but well-earned trip to the sun, and before she left she made the H. influenzae hfq knockout I've been waiting for. So yesterday, after clicking 'Submit' on our NSERC grant proposal, I did competence time courses of wildtype cells and the mutant, hoping to see a difference in transformation frequencies (preferably a decrease).
You can read all the background in this post from last March. And here's a hexameric structure for Hfq (from Wikipedia):
The colonies are still too tiny to count, but it looks like the transformation frequency is down about 10-fold in the mutant!
Next step, make some DNA from the mutant so I can introduce the mutation into my hypercompetent mutant backgrounds.
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