I would normally set up four cultures (wildtype, toxin knockout, antitoxin knockout, double knockout), but there's a complication. The double knockout antitoxin mutant only exists in a 'marked' version (with a spectinomycin cassette inserted in place of the missing genes) and the antitoxin knockout only exists in an 'unmarked' version (no spectinomycinR cassette). If this cassette influences growth or survival this difference could cause anomalous results. The toxin knockout exists in both forms, so I'll include both of them in the analysis.
First step is to restreak all the strains from the freezer stock. I did this last week but foolishly let the cells die on the plates rather than restreaking them.