I just discovered a 2008 paper in Mutation Research, about the phenotypes of H. influenzae exonuclease mutants, and I've emailed the senior author asking if they would be willing to send us chromosomal DNA of these mutants, so we could test a pet hypothesis of mine.
The hypothesis concerns the functions of the competence-induced genes comM and dprA. Phenotypes of mutants in other bacteria suggest that the products of these genes protect incoming DNA from nuclease degradation. But what nuclease? By testing the competence phenotypes of double mutants we've ruled out ExoV (recBCD). Using the new mutants would let us test the involvement of other nucleases. (The hypothesis is described more thoroughly in this blog post.)
A 2002 paper about Snyechocystis shows that knocking out recJ increases transformation frequencies 100-fold. Synechocycsis does have homologs of both comM and dprA, so I don't know what the increased transformation implies about their roles.
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Field of Science
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RFK Jr. is not a serious person. Don't take him seriously.2 months ago in Genomics, Medicine, and Pseudoscience
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The Site is Dead, Long Live the Site2 years ago in Catalogue of Organisms
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The Site is Dead, Long Live the Site2 years ago in Variety of Life
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What I read 20194 years ago in Angry by Choice
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Histological Evidence of Trauma in Dicynodont Tusks6 years ago in Chinleana
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Posted: July 21, 2018 at 03:03PM6 years ago in Field Notes
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Why doesn't all the GTA get taken up?6 years ago in RRResearch
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Harnessing innate immunity to cure HIV8 years ago in Rule of 6ix
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post doc job opportunity on ribosome biochemistry!9 years ago in Protein Evolution and Other Musings
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Blogging Microbes- Communicating Microbiology to Netizens10 years ago in Memoirs of a Defective Brain
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Re-Blog: June Was 6th Warmest Globally10 years ago in The View from a Microbiologist
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The Lure of the Obscure? Guest Post by Frank Stahl12 years ago in Sex, Genes & Evolution
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Lab Rat Moving House13 years ago in Life of a Lab Rat
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Goodbye FoS, thanks for all the laughs13 years ago in Disease Prone
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Slideshow of NASA's Stardust-NExT Mission Comet Tempel 1 Flyby13 years ago in The Large Picture Blog
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in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
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