The good news is that my test transformation worked quite well - the transformation frequency was a bit lower than I would like, but the numbers of colonies produced from the different dilutions were just what they should have been, and the colonies themselves were both vigorous and uniform.
The bad news is that our work-study student has the flu, so I won't have any help with the big experiment. But of course it isn't really that big of an experiment, just a full day of keeping track and paying attention and neglecting other responsibilities.
The Litiopids: Small Sea-Snails among the Weeds
6 hours ago in Catalogue of Organisms