Nothing that has generated any big insights. The RA and I are working on the E. coli competence question: Given that E. coli appears to have all the genes needed for DNA uptake and transformation, can we detect competence or transformation? We have several strategies: 1. Artificially induce sxy expression to turn on the competence genes, using one of two IPTG-inducible sxy plasmid constructs. 2. Use a recombineering protein to increase the efficiency of recombination. 3. Screen strains of the ECOR collection.