Why H. influenzae and competence are important.
- Gap 1: Which proteins affect which steps in uptake? [Here make the point that the steps themselves are not defined yet.]
- Gap. 2: What is the real sequence specificity and how does it contribute to DNA uptake?
- Gap. 3: What proteins exert forces on the DNA?
Specific Aims: (5 questions, perhaps grouped into 'Aims')
- Q. 1,
- Q. 2,
- Q. 3,
- Q. 4,
- Q. 5
Q. 1: Which genes affect which steps of DNA uptake?
Q. 2: Which genes cause the different uptake specificities of H. influenzae and A. pleuropneumoniae?*
Q. 3: What are the uptake specificities of H.influenzae and A. pleuropneumoniae?*
Q. 4: What are the physical properties of the H. influenzae and A. pleuropneumoniae USSs?
Q. 5: What forces do cells exert on DNA during uptake, and which genes are needed for these forces?
* The reader will ask why Q. 3 comes after Q. 2 when Q. 3 should be answered before Q.2 can be answered. It depends on how/if the questions are organized into Aims. (Otherwise we could just change the 'Specific Aims' heading to 'Specific Questions to be answered'.