Now I just want to confirm that the recombinant strain does have an intermediate phenotype, and, more generally, to carefully check the phenotypes of both parents and the recombinant, under all of the conditions we know that affect competence. So I want to check competence at all stages of culture growth in rich medium (a detailed time course) and after transfer to the starvation medium MIV.
Below are the results of a simple time-course experiment using cells growing in rich medium. It's not a detailed time course since I only did 4 time points, so I plotted transformation frequency as a function of cell density rather than of time, but it clearly shows that the recombinant has an intermediate phenotype.
Next I'll do a better time course and MIV-induced competence. I should also test induction of competence by transient shift to anaerobic culture, and by addition of cAMP to log-phase cells.