Field of Science

My work is laid out for me

I have to make competent cell preparations of a lot of our knockout mutants, and do transformation assays on almost all of them.  They're mostly replicates of ones I've already done at least once, so I know what transformation frequency to expect (not so I can fudge the data, but so I can optimize the dilutions I plate to get the most information.

I also have to freeze four tubes of each competent preparation so the postdoc can use them in DNA uptake assays.  He thoughtfully had one of the undergrads prepare all the freezer tubes for me (label them and add 250 µl of 80% glycerol).

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