Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
My clever strategy for making a double knockout of the HI0659 and HI0660 genes has been derailed by the absence of plasmid in one of the E. coli strains and the absence of the SpcR resistance cassette from the other strain's plasmid. I suspect that I've been given the wrong strains...
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