The Co-op tech will be leaving us at the end of the month. Last week she gave an excellent bab meeting presentation, and this revealed a couple of loose ends and interesting possibilities that should be cleared up before she leaves.
First, she and the sabbatical visitor (now back home in Regina) isolated a new mutation in rpoD that causes hypercompetence. But this strain hasn't yet been added to our formal collection of frozen strains and the associated 'Strain List' database. This is essential and urgent.
Second, the mutant hunt turned up a couple of other 'possibly hypercompetent' mutants whose phenotypes haven't been checked out. These need to be checked in competence time courses, and added to the Strain List collection if they turn out to be genuinely hypercompetent.
A weird result that I'd forgotten about is the finding that cells transformed with a mixture of NovR and KanR DNA fragments (generated by PCR) showed a much LOWER cotransformation frequency than expected. We suspect this reflects some chromosome-level interaction between these linked segments, but we'd first need to reproduce the result.
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Field of Science
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Hivestorm1 year ago in Pleiotropy
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The Site is Dead, Long Live the Site1 year ago in Catalogue of Organisms
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The Site is Dead, Long Live the Site1 year ago in Variety of Life
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Does mathematics carry human biases?3 years ago in PLEKTIX
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Daily routine3 years ago in Angry by Choice
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A New Placodont from the Late Triassic of China4 years ago in Chinleana
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Posted: July 22, 2018 at 03:03PM5 years ago in Field Notes
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Bryophyte Herbarium Survey6 years ago in Moss Plants and More
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Harnessing innate immunity to cure HIV7 years ago in Rule of 6ix
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WE MOVED!7 years ago in Games with Words
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post doc job opportunity on ribosome biochemistry!8 years ago in Protein Evolution and Other Musings
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Growing the kidney: re-blogged from Science Bitez8 years ago in The View from a Microbiologist
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Blogging Microbes- Communicating Microbiology to Netizens9 years ago in Memoirs of a Defective Brain
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The Lure of the Obscure? Guest Post by Frank Stahl11 years ago in Sex, Genes & Evolution
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Lab Rat Moving House12 years ago in Life of a Lab Rat
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Goodbye FoS, thanks for all the laughs12 years ago in Disease Prone
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Slideshow of NASA's Stardust-NExT Mission Comet Tempel 1 Flyby12 years ago in The Large Picture Blog
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in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
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