The Co-op tech will be leaving us at the end of the month. Last week she gave an excellent bab meeting presentation, and this revealed a couple of loose ends and interesting possibilities that should be cleared up before she leaves.
First, she and the sabbatical visitor (now back home in Regina) isolated a new mutation in rpoD that causes hypercompetence. But this strain hasn't yet been added to our formal collection of frozen strains and the associated 'Strain List' database. This is essential and urgent.
Second, the mutant hunt turned up a couple of other 'possibly hypercompetent' mutants whose phenotypes haven't been checked out. These need to be checked in competence time courses, and added to the Strain List collection if they turn out to be genuinely hypercompetent.
A weird result that I'd forgotten about is the finding that cells transformed with a mixture of NovR and KanR DNA fragments (generated by PCR) showed a much LOWER cotransformation frequency than expected. We suspect this reflects some chromosome-level interaction between these linked segments, but we'd first need to reproduce the result.
- Home
- Angry by Choice
- Catalogue of Organisms
- Chinleana
- Doc Madhattan
- Games with Words
- Genomics, Medicine, and Pseudoscience
- History of Geology
- Moss Plants and More
- Pleiotropy
- Plektix
- RRResearch
- Skeptic Wonder
- The Culture of Chemistry
- The Curious Wavefunction
- The Phytophactor
- The View from a Microbiologist
- Variety of Life
Field of Science
-
-
Earth Day: Pogo and our responsibility2 months ago in Doc Madhattan
-
What I Read 20243 months ago in Angry by Choice
-
I've moved to Substack. Come join me there.4 months ago in Genomics, Medicine, and Pseudoscience
-
-
The Site is Dead, Long Live the Site2 years ago in Catalogue of Organisms
-
The Site is Dead, Long Live the Site2 years ago in Variety of Life
-
-
-
-
Histological Evidence of Trauma in Dicynodont Tusks6 years ago in Chinleana
-
Posted: July 21, 2018 at 03:03PM6 years ago in Field Notes
-
Why doesn't all the GTA get taken up?7 years ago in RRResearch
-
-
Harnessing innate immunity to cure HIV8 years ago in Rule of 6ix
-
-
-
-
-
-
post doc job opportunity on ribosome biochemistry!10 years ago in Protein Evolution and Other Musings
-
Blogging Microbes- Communicating Microbiology to Netizens10 years ago in Memoirs of a Defective Brain
-
Re-Blog: June Was 6th Warmest Globally10 years ago in The View from a Microbiologist
-
-
-
The Lure of the Obscure? Guest Post by Frank Stahl13 years ago in Sex, Genes & Evolution
-
-
Lab Rat Moving House13 years ago in Life of a Lab Rat
-
Goodbye FoS, thanks for all the laughs13 years ago in Disease Prone
-
-
Slideshow of NASA's Stardust-NExT Mission Comet Tempel 1 Flyby14 years ago in The Large Picture Blog
-
in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment
Markup Key:
- <b>bold</b> = bold
- <i>italic</i> = italic
- <a href="http://www.fieldofscience.com/">FoS</a> = FoS