I've scored all my transformation plates and found no evidence of transformation. The Tet plates have no colonies at all. This could be because I used 20 µg/ml when I should have used 10; I'm checking the resistance of RR902 to see if I should repeat this transformation. The Kan plates had lots of colonies when cells were plated undiluted, but the numbers were similar for cells with and without DNA. With DH5alpha the colonies were all tiny even after two days, but quite a few of the colonies produced by the BW25113 derivative RR3015 were reasonably large.
What next? The RA is also going to repeat these experiments, using exactly the same method that gave apparent transformants previously. And I'm going to streak some of my KanR colonies onto MacConkey maltose (the recipeints are all Lac-) to see if any are crp- as true transformants should be.
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Earth Day: Pogo and our responsibility2 months ago in Doc Madhattan
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What I Read 20243 months ago in Angry by Choice
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I've moved to Substack. Come join me there.4 months ago in Genomics, Medicine, and Pseudoscience
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The Site is Dead, Long Live the Site2 years ago in Catalogue of Organisms
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The Site is Dead, Long Live the Site2 years ago in Variety of Life
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Histological Evidence of Trauma in Dicynodont Tusks6 years ago in Chinleana
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Posted: July 21, 2018 at 03:03PM6 years ago in Field Notes
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Why doesn't all the GTA get taken up?7 years ago in RRResearch
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Harnessing innate immunity to cure HIV8 years ago in Rule of 6ix
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post doc job opportunity on ribosome biochemistry!10 years ago in Protein Evolution and Other Musings
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Blogging Microbes- Communicating Microbiology to Netizens10 years ago in Memoirs of a Defective Brain
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Re-Blog: June Was 6th Warmest Globally10 years ago in The View from a Microbiologist
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The Lure of the Obscure? Guest Post by Frank Stahl13 years ago in Sex, Genes & Evolution
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Lab Rat Moving House13 years ago in Life of a Lab Rat
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Goodbye FoS, thanks for all the laughs13 years ago in Disease Prone
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Slideshow of NASA's Stardust-NExT Mission Comet Tempel 1 Flyby14 years ago in The Large Picture Blog
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in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
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