The streptavidin-coated magnetic beads that hadn't been blocked with BSA aggregated into big clumps when mixed with cells, but the treated beads remained separate. The clumping was independent of whether the beads had been incubated with biotinylated DNA, so I think the clumping was because most cells stuck to more than one bead and most beads stuck to more than one cell.
So then I checked the streptavidin-coated polystyrene beads I'll use for the tweezers experiments. Luckily adding cells didn't make them clump.
Now I'm making fresh batches of competent H. influenzae and B. subtilis to freeze, so I'll have consistent cells to work with.
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Posted: July 22, 2018 at 03:03PM6 years ago in Field Notes
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post doc job opportunity on ribosome biochemistry!9 years ago in Protein Evolution and Other Musings
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in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
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