Yesterday I scored the results of Wednesday's test. I had incubated competent H. influenzae cells with magnetic beads, using both DNA-coated beads and control beads that had gone through the same treatment without DNA. Then I had washed the unattached cells away from the beads, taking samples for plating at every step so I could track the progress of the three washes. I added DNase I to the beads after the last wash, so I'd be counting the total number of attached cells rather than the number of beads that had cells on them. Then I plated all the samples, and later counted the colonies.
There were ten times more cells on the DNA-coated beads than on the control beads (about 10^5 vs 10^4). This is good, but because I used about 10^8 beads, it means that, at best, only about one bead in 1000 had a cell attached. I hadn't measured how much DNA was on these beads, lazily hoping that they had as much DNA as the similarly treated polystyrene beads. Now I need to repeat the experiment with better-characterized beads.
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in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
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