Field of Science

We now resume our #arseniclife blogging...

When we paused last week, I had just noted the unexpected result that, in two of three tests (#1184 and #1189), GFAJ-1 cells in 3 µM PO4 + 40 mM AsO4 grew to a higher density  than the cells in 3 µM PO4 (no AsO4), but the cells with more and with less phosphate didn't grow much at all.  In the other test (#1188) the cells didn't grow at all in the arsenic cultures; I won't say anything more about these cultures unless I get more results like this.  All cultures without arsenic grew as they had in previous experiments.

I left the #1189 arsenic cultures in the incubator while I was away, and over this ~225 hr incubation the 1.5 mM-phosphate' and no-added-phosphate cultures grew to densities close to those of their no-arsenate controls.  I don't know why they grew so much slower than the 3 µM phosphate+arsenate culture.

I'm going to repeat these cultures once more.  If I see the same results (fast high growth of cells in 3 µM phosphate + arsenate), I'll dilute the 3 µM phosphate cultures into 400 ml of the same medium ± arsenate, to get lots of cells for my first arsenate-grown DNA prep.

1 comment:

  1. Hi, your "lab-blogging" is very interesting, thanks for that ! From a french post-PhD ;)


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