To help me figure out what nutrient might be missing from the AML60 medium I'm trying to grow GFAJ-1 in, I'm reading about its Halomonas relatives.
Here's a tree showing the relationship between GFAJ-1 and its closest known relatives (source).
And here's a link to a paper describing VERY THOROUGH phenotypic characterization of all the formally described species of Halomonas: Mata et al., 2002. A detailed phenotypic characterization of the type strains of Halomonas species. System. Appl. Microbiol. 25:360-375. I was initially assuming that GFAJ-1 would be quite picky about growth conditions, but if it's a typical Halomonas species it's probably quite robust, as they all tolerate wide ranges of salt concentration, pH and temperature.
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