Field of Science

Growth properties of GFAJ-1's relatives?

To help me figure out what nutrient might be missing from the AML60 medium I'm trying to grow GFAJ-1 in, I'm reading about its Halomonas relatives.

Here's a tree showing the relationship between GFAJ-1 and its closest known relatives (source).

And here's a link to a paper describing VERY THOROUGH phenotypic characterization of all the formally described species of Halomonas:  Mata et al., 2002.  A detailed phenotypic characterization of the type strains of Halomonas species.  System. Appl. Microbiol. 25:360-375.  I was initially assuming that GFAJ-1 would be quite picky about growth conditions, but if it's a typical Halomonas species it's probably quite robust, as they all tolerate wide ranges of salt concentration, pH and temperature.

1 comment:

  1. Regarding that figure, I am one of the wikipedia editors for the English version of GFAJ-1 and I though it may be of interest to mention that the other editors and I had a behind the scene discussion on what to state on the page due to the "information [being] incorrect by omission". Doing some background research it was clear that
    — GFAJ-1 is definitely a Halomonas sp.
    — the 16S sequence is over 99% similar to stain GTW (paper)
    — the fact this group of strain does not have an official standing is because nobody has cared to officially describe them, not because they are distant/ancient Halomonaceae (as the paper hints)


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