To help me figure out what nutrient might be missing from the AML60 medium I'm trying to grow GFAJ-1 in, I'm reading about its Halomonas relatives.
Here's a tree showing the relationship between GFAJ-1 and its closest known relatives (source).
And here's a link to a paper describing VERY THOROUGH phenotypic characterization of all the formally described species of Halomonas: Mata et al., 2002. A detailed phenotypic characterization of the type strains of Halomonas species. System. Appl. Microbiol. 25:360-375. I was initially assuming that GFAJ-1 would be quite picky about growth conditions, but if it's a typical Halomonas species it's probably quite robust, as they all tolerate wide ranges of salt concentration, pH and temperature.
Field of Science
-
-
-
-
-
-
-
-
The Even Earlier Discovery of Antibiotic Resistance2 days ago in Memoirs of a Defective Brain
-
Religion is halfway between a fact and an opinion - according to kids and adults4 days ago in Epiphenom
-
Bioengineers go retro to build a calculator from living cells4 days ago in The Allotrope
-
-
A New Non-mammaliaform Eucynodont from the Ischigualasto Formation of Argentina1 week ago in Chinleana
-
-
Chemistry, fluid dynamics and an awful radioactive mess2 weeks ago in The Curious Wavefunction
-
Exploding expertise2 weeks ago in The Culture of Chemistry
-
-
-
-
-
-
-
-
-
The Lure of the Obscure? Guest Post by Frank Stahl11 months ago in Sex, Genes & Evolution
-
-
Finding a new translation factor, and verifying it with help from my experimental friends1 year ago in Protein Evolution and Other Musings
-
Free ImageJ Macro -- for citing images1 year ago in Skeptic Wonder
-
-
-
The Large Picture Blog Has Moved1 year ago in The Large Picture Blog
-
Lab Rat Moving House1 year ago in Life of a Lab Rat
-
Goodbye FoS, thanks for all the laughs1 year ago in Disease Prone
-
Branson getting into microbial diversity in the deep sea2 years ago in The Greenhouse
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
1 comment:
Markup Key:
- <b>bold</b> = bold
- <i>italic</i> = italic
- <a href="http://www.fieldofscience.com/">FoS</a> = FoS
Subscribe to:
Post Comments (Atom)

Regarding that figure, I am one of the wikipedia editors for the English version of GFAJ-1 and I though it may be of interest to mention that the other editors and I had a behind the scene discussion on what to state on the page due to the "information [being] incorrect by omission". Doing some background research it was clear that
ReplyDelete— GFAJ-1 is definitely a Halomonas sp.
— the 16S sequence is over 99% similar to stain GTW (paper)
— the fact this group of strain does not have an official standing is because nobody has cared to officially describe them, not because they are distant/ancient Halomonaceae (as the paper hints)