First, GFAJ-1 cells grow well in liquid AML60 medium supplemented with 10 mM glutamate.
Second, they don't grow much at all if I omit the phosphate from the AML60 medium.
Together this means that I now have conditions for investigating whether the cells can incorporate arsenic into their DNA when phosphate is limiting.
But I first need to now do some careful growth curves. This will be relatively easy because I've realized that Halomonas are nutritionally quite versatile, and I can get good colonies on agar plates overnight if I supplement the AML60 medium with tryptone or yeast extract.