The computer cluster guys were great. They took extra pains to make sure we understood the big issues, they were really helpful in suggesting ways to optimize our runs, and they even gave us a tour of the WestGrid system.
I've cataloged the reverse-complement runs (same basic results as the forward runs), and made this composite logo based on all the USSs in the genome (i.e. both strands). It doesn't look any different than a composite logo I posted about 6 weeks ago; the difference here is that now I know I've done the right analysis.
Now on to analyzing the leading-strand and lagging-strand searches. I already did a quick-and-dirty version of that too, but again now I'll have done it well.
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RFK Jr. is not a serious person. Don't take him seriously.1 month ago in Genomics, Medicine, and Pseudoscience
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The Site is Dead, Long Live the Site2 years ago in Catalogue of Organisms
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The Site is Dead, Long Live the Site2 years ago in Variety of Life
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What I read 20194 years ago in Angry by Choice
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post doc job opportunity on ribosome biochemistry!9 years ago in Protein Evolution and Other Musings
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in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
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Nice logo - your "unbiased" analysis really brings out the AT runs 3' to the 9bp USS core. How many USSs (or G-USSs?) did you find? How many are 1 off and 2 off?
ReplyDeleteScratch that. I thought I had read your previous post, but now I see that it has all the answers.
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