A few days ago I posted an old graph of transformation data that appeared to show that the DNA-protection gene dprA was not needed in cells whose recBC gene was knocked out. This was a result I didn't remember getting, so I was going to search through my old notebook (2003-04) to see if this was a real result.
This experiment turned out to not be in my notebook because it was done by an undergraduate working in the lab. She did good work, but I can't find her lab notes on our shelf of old notebooks. (I hereby vow to work with our new lab assistant to get these notebooks better labeled and organized.) My notes of her lab meeting presentations and other discussions don't directly address this point.
But I gradually remembered that her initial results had been wrong (maybe that the strain she thought was a double dprA recBC mutant was actually only a single recBC mutant?), and that correcting the error had eliminated this interesting result, replacing it with a more solid but less interesting one. I did find a copy of her final report . It shows clearly that the double mutant had the same transformation phenotype as the single dprA mutant, so she concluded that DprA's job is not to inhibit the nuclease activity of RecBC.
- Home
- Angry by Choice
- Catalogue of Organisms
- Chinleana
- Doc Madhattan
- Games with Words
- Genomics, Medicine, and Pseudoscience
- History of Geology
- Moss Plants and More
- Pleiotropy
- Plektix
- RRResearch
- Skeptic Wonder
- The Culture of Chemistry
- The Curious Wavefunction
- The Phytophactor
- The View from a Microbiologist
- Variety of Life
Field of Science
-
-
-
-
Hivestorm1 year ago in Pleiotropy
-
The Site is Dead, Long Live the Site1 year ago in Catalogue of Organisms
-
The Site is Dead, Long Live the Site1 year ago in Variety of Life
-
-
Does mathematics carry human biases?3 years ago in PLEKTIX
-
-
-
Daily routine3 years ago in Angry by Choice
-
-
A New Placodont from the Late Triassic of China4 years ago in Chinleana
-
Posted: July 22, 2018 at 03:03PM5 years ago in Field Notes
-
Bryophyte Herbarium Survey6 years ago in Moss Plants and More
-
Harnessing innate immunity to cure HIV7 years ago in Rule of 6ix
-
WE MOVED!7 years ago in Games with Words
-
-
-
-
post doc job opportunity on ribosome biochemistry!8 years ago in Protein Evolution and Other Musings
-
Growing the kidney: re-blogged from Science Bitez8 years ago in The View from a Microbiologist
-
Blogging Microbes- Communicating Microbiology to Netizens9 years ago in Memoirs of a Defective Brain
-
-
-
The Lure of the Obscure? Guest Post by Frank Stahl11 years ago in Sex, Genes & Evolution
-
-
Lab Rat Moving House12 years ago in Life of a Lab Rat
-
Goodbye FoS, thanks for all the laughs12 years ago in Disease Prone
-
-
Slideshow of NASA's Stardust-NExT Mission Comet Tempel 1 Flyby12 years ago in The Large Picture Blog
-
in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
No comments:
Post a Comment
Markup Key:
- <b>bold</b> = bold
- <i>italic</i> = italic
- <a href="http://www.fieldofscience.com/">FoS</a> = FoS