Field of Science

Growing cells and making RNA (continued)

On Thursday and Friday I finally was able to collect cells from cultures of wildtype H. influenzae cells growing happily (exponentially) in medium with and without a sub-inhibitory concentration of rifampcin. On Friday I did a RNA prep of the cells I'd collected on Thursday, working with our new undergraduate assistant. Yesterday the undergrad did the RNA preps of Friday's cells by herself.

And tomorrow, she and I will run a gel to check that the RNAs are in good shape (not degraded), treat them with DNA-Free, and pass them on to our collaborators for real-time PCR analysis.

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