for GFAJ-1, that is. (I have plenty of excuses for myself.)
My favourite old-timer microbiologist had lots of PABA and was happy to give me some. And, after some fussing with the pH to get the folic acid to dissolve, I now have my sterile 500x multi-vitamin stock solution for the specified ML60 culture medium. And I also have the 1000x trace element solution, thanks to a grad student in another colleague's lab. So now I can make medium exactly as specified for GFAJ-1 cells to grow in.
I also have fresh single colonies of cells to resuspend and inoculate into this medium. I'll use the Petroff-Hausser counter and acridine orange staining to count the number of cells the new cultures start with,and recount them every day.
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Field of Science
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Don't tell me they found Tyrannosaurus rex meat again!2 weeks ago in Genomics, Medicine, and Pseudoscience
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Course Corrections4 months ago in Angry by Choice
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The Site is Dead, Long Live the Site2 years ago in Catalogue of Organisms
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The Site is Dead, Long Live the Site2 years ago in Variety of Life
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Does mathematics carry human biases?4 years ago in PLEKTIX
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A New Placodont from the Late Triassic of China5 years ago in Chinleana
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Posted: July 22, 2018 at 03:03PM6 years ago in Field Notes
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Bryophyte Herbarium Survey7 years ago in Moss Plants and More
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Harnessing innate immunity to cure HIV8 years ago in Rule of 6ix
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WE MOVED!8 years ago in Games with Words
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post doc job opportunity on ribosome biochemistry!9 years ago in Protein Evolution and Other Musings
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Growing the kidney: re-blogged from Science Bitez9 years ago in The View from a Microbiologist
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Blogging Microbes- Communicating Microbiology to Netizens10 years ago in Memoirs of a Defective Brain
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The Lure of the Obscure? Guest Post by Frank Stahl12 years ago in Sex, Genes & Evolution
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Lab Rat Moving House13 years ago in Life of a Lab Rat
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Goodbye FoS, thanks for all the laughs13 years ago in Disease Prone
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Slideshow of NASA's Stardust-NExT Mission Comet Tempel 1 Flyby13 years ago in The Large Picture Blog
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in The Biology Files
Not your typical science blog, but an 'open science' research blog. Watch me fumbling my way towards understanding how and why bacteria take up DNA, and getting distracted by other cool questions.
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