But first we need to replicate our results. My plan is to generate some detailed growth curves for cultures with various levels of phosphate, with and without 40 mM arsenate. For this I'll use a BioScreen machine that belongs to a neighbouring lab. This machine automates collection of optical density data from cultures growing in wells of 100-well plates. I'll also grow big batches of cells for new DNA preps, using the same media and culture conditions as before.
This should only take a few days, and I hope to have the DNAs ready to send to my collaborators on Monday.