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Why did I put SDS into the buffer of this agarose gel before I loaded it? So the DNA from the lysed cells wouldn't rise up out of the wells and spread out over the surface of the gel buffer, of course!
I'll tell you more tomorrow, if my experiment works out
I'm curious how did it work out?
ReplyDeleteI avoid this problem by loading the gel and running the samples in for about 20 minutes in a 'non-submarine' manner (i.e. with the running buffer making electrical contact with the ends of the gel but not overlaying it). Once the samples have migrated into the gel, it's safe to add more buffer and run submarine.
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