for GFAJ-1, that is. (I have plenty of excuses for myself.)
My favourite old-timer microbiologist had lots of PABA and was happy to give me some. And, after some fussing with the pH to get the folic acid to dissolve, I now have my sterile 500x multi-vitamin stock solution for the specified ML60 culture medium. And I also have the 1000x trace element solution, thanks to a grad student in another colleague's lab. So now I can make medium exactly as specified for GFAJ-1 cells to grow in.
I also have fresh single colonies of cells to resuspend and inoculate into this medium. I'll use the Petroff-Hausser counter and acridine orange staining to count the number of cells the new cultures start with,and recount them every day.
Information and Structure in Complex Systems
21 hours ago in PLEKTIX