- Analysis of the true consensus and variation in uptake sequence motifs in all the bacterial genomes that have uptake sequences (= the family Pasteurellaceae (USS) and the genus Neisseria (DUS)).
- Analysis of variation in DUS and USS motifs across different location categories (in coding sequences, in non-coding sequences, in terminator positions).
- Analysis of covariation between the different positions of the DUS and USS uptake sequence motifs (e.g. does having a particular base at one position correlate with having a particular base at another position).
- Additional experimental data on how variation in uptake sequence affects uptake by H. influenzae. (This will just be a paragraph as it only modestly enriches a previously published dataset.)
- Development of a computer-simulation model of uptake sequence evolution, and use of it to investigate the roles of key factors in maintaining uptake sequences in the non-coding parts of genomes.
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