What strains will I test? The two strains that she has previously tested, plus a control strain that doesn't carry the plasmid we use to induce the chromosomal competence genes. (I could not bother with the DH5alpha strain, but I think its unexpected phenotype may provide useful information.)
What DNA will I use? The RA has made a stock of DNA from a strain carrying a kanamycin-resistance insertion in the crp gene. She uses it at 2 micrograms/ml. She's now making a better strain whose DNA we'll use for future experiments; it carries this insertion and easily-selectable wild-type alleles of other genes.
How will I prepare the cells? I've grown the two plasmid-carrying cultures overnight from single colonies (over two nights really as I inoculated them on Friday night). I'll dilute these 1/100 in LB and grow them to OD=0.2 The other strain I'll need to inoculate directly from a single colony this morning. When the cells are at OD=0.2 I'll add IPTG and then the DNA. After 2 hours I'll plate the cells with and without kanamycin. I'll use a wide range of dilutions on the kanamycin plates, because the RA has found 'bald-spot' problems when dense cultures are plated directly. I'll include a negative control with no DNA, and another with DNaseI added at the same time as the DNA.
What do I need to do to prepare? Pour lots of LB plates, especially kanamycin ones.