Field of Science

Photo documentation

 This is an iphone snap of my test plate after overnight incubation.  The first and third streaks are a recA+ control strain and the second and fourth are of a known recA- strain.  Because I was working at an odd angle, the green lines I drew to guide the exposures aren't lined up with the actual exposures.   The recA+ cells grew fine after 5 seconds of UV, and gave a few colonies even after 1 minute (probably cells that were shielded in some way from the UV).  The recA- cells grew fine when they were not exposed to any UV, but didn't grow at all even after only 5 seconds UV.

So today I've streaked all the cells I want to test, each on at least 2 different plates.  I also reduced the UV dose.  Yesterday I used a high dose range (5, 15, 30 and 60 seconds) to make sure I had a dose that would kill the recA- cells; today I used 2, 5 10 and 15 seconds.

1 comment:

  1. Experiments are always sexiest with a picture! Thanks to share it.


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